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. 2016 Jul 28;11:350. doi: 10.1186/s11671-016-1558-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — The binding of free J3/J3 linked to liposomes to recombinant gp140 (CN54) was compared in ELISA. Gp140 was immobilized on the plate, and Vhh was detected with anti-myc-HRP. Irrelevant Vhh (lab5) and liposome-irrelevant Vhh were included as negative controls. Values are means ± standard deviations of three replicate measurements in one representative experiment of three independent experiments. *From one-way ANOVA, significant differences were observed (p < 0.001) between lipo3-J3 and the other liposome formulations or free Vhhs