Table 1.
Characterisation of the immunoliposomes
| Liposome | PC conc. (mg/ml)a | Dapivirine (μM) | No. of Vhh molecules per liposome | Vhh (μg/ml) | Diameter (nm)a | Zeta potential (mV)a |
|---|---|---|---|---|---|---|
| Lipo1-lab5 | 11.937 ± 0.001 | – | 160 | 126.3 | 228.0 ± 1.0 | −45.0 ± 0.9 |
| Lipo1-J3 | 11.708 ± 0.008 | – | 160 | 118.4 | 223.4 ± 4.5 | −37.4 ± 0.5 |
| Lipo2-lab5 | 5.974 ± 0.005 | – | 95 | 150.0 | 130.9 ± 1.4 | −38.7 ± 0.7 |
| Lipo2-J3 | 6.149 ± 0.003 | – | 108 | 159.9 | 131.2 ± 1.9 | −38.5 ± 0.2 |
| Lipo2-dapi | 6.478 ± 0.001 | 6.5 | – | – | 122.9 ± 1.8 | −46.0 ± 0.9 |
| Lipo2-dapi-J3 | 5.548 ± 0.002 | 5.5 | 125 | 169.6 | 123.4 ± 1.8 | −38.5 ± 0.4 |
| Lipo3 control | 7.573 ± 0.003 | – | – | – | 130.9 ± 1.7 | −42.5 ± 0.4 |
| Lipo3-J3 | 6.456 ± 0.004 | – | 121 | 168.2 | 142.2 ± 3.2 | −36.4 ± 0.4 |
| Lipo3-dapi | 6.675 ± 0.003 | 6.7 | – | – | 128.2 ± 2.6 | −41.7 ± 0.5 |
| Lipo3-dapi-J3 | 6.806 ± 0.001 | 6.8 | 108 | 157.0 | 139.1 ± 1.8 | −37.8 ± 0.6 |
Dapi: dapivirine
aData are means ± standard deviations of three replicate measurements in one representative experiment of three independent experiments