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. 2016 Jul 28;7:1156. doi: 10.3389/fmicb.2016.01156

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Copyright © 2016 Blázquez, Fresco-Taboada, Iglesias-Bexiga, Menéndez and García.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of choline binding on PL3 structure. Comparison of the far- (A) and near-UV (B) CD spectra of PL3, Pal, and LytA in the absence and in the presence of 20 mM choline. (C) Effect of choline binding on PL3 association-state distribution. Increase of choline concentration shifts the monomer/dimer/tetramer equilibria of PL3 toward the dimer (s_20,w = 4.1 S) with the concomitant decrease of monomer (s_20,w = 3.0) and tetramer (s_20,w = 6.8 S) populations. Labels indicate the choline concentration (mM). (D) CD titration curve of PL3 (squares) generated from the ellipticity changes induced at 295 nm by choline binding. Titration curves of LytA (circles; Varea et al., 2000) and Pal (triangles; Varea et al., 2004) are also depicted. Continuous lines represent the fitting of experimental data as the sum of two sigmoids with the parameters shown in Supplementary Table S1.