Table 1.
Bacterial strains and plasmids.
| Strains or plasmids | Genotype or descriptiona | Referenceb |
|---|---|---|
| Strains | ||
| Escherichia coli BL21(DE3) | F-, ompT, hsdSB (rB-mB-), gal, dcm, aaaaDE3 (harboring gene 1 of the RNA polymerase from the phage T7 under the PlacUV5 promoter) | Sambrook and Russell, 2001 |
| Streptococcus pneumoniae | ||
| R6 | Standard laboratory strain, non-encapsulated | Hoskins et al., 2001 |
| P046 | R6 but lytA, lytC | Moscoso et al., 2006 |
| D39 | Serotype 2 | Lanie et al., 2007 |
| P007 | R6 derivative, serotype 3 | Domenech et al., 2009 |
| P008 | R6 derivative, serotype 4 | Moscoso et al., 2006 |
| 48 | Serotype 23F; penicillin MIC = 16 mg/ml; erythromycin MIC > 128 mg/ml; ciprofloxacin MIC = 1 mg/ml; levofloxacin MIC = 1 mg/ml; chloramphenicol MIC = 4 mg/ml; tetracycline MIC > 64 mg/ml | Soriano et al., 2008 |
| Streptococcus mitisT | Type strain | NCTC 12261 |
| Streptococcus mitis SK598 | Biovar 1 strain with ethanolamine-containing C-polysaccharide | Bergström et al., 2003 |
| Streptococcus oralisT | Type strain | NCTC 11427 |
| Streptococcus pseudopneumoniaeT | Type strain | ATCC BAA-960 |
| Plasmids | ||
| pET29a(+) | Expression vector; KmR | Novagen |
| pET29-PL3 | pET29a(+), pl3; KmR | This study |
| pMSP11 | Recombinant plasmid with pal | Sheehan et al., 1997 |
| pMMN1 | Recombinant plasmid with lytA | Moscoso et al., 2011 |
aMIC, minimal inhibitory concentration.
bNCTC, National Collection of Type Cultures; ATCC, American Type Culture Collection.