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Pipetting scheme for quantitative real-time PCR.
| Reagent | Volume (μl) |
|---|---|
| Template plasmid (50–100 ng)/standard/double-distilled water (negative control) | 5,0 |
| KAPA SYBR FAST Universal x2 qPCR MasterMix | 10,0 |
| WPREqF (10 pmol/μl) | 1,0 |
| WPREqR (10 pmol/μl) | 1,0 |
| Double-distilled water | ad 20,0 |
