Table 3.

Program steps of quantitative real-time PCR for titer determination.

Step	Phase	Temperature (°C)	Time	Cycle numbers
1	Initial Denaturation	95	10 min	1
2	Denaturation	95	10 s	40
3	Annealing	60	5 s
4	Elongation	72	20 s
5	Final elongation	72	5 min	1