Fig. 2.

Platelet coverage and fibrin formation on the fixed endothelium in the microdevice. a Representative maximum intensity projection micrographs showing fluorescently labeled platelets adhering to the fixed endothelium in a tumor necrosis factor (TNF-α) dose-dependent manner (bar, 100 μm). b Graph showing platelet coverage when blood is perfused inside the microchannel lined with a living or fixed endothelium that was stimulated by TNF-α before fixation. Comparison of living vs. fixed microchannels was not significantly different (P > 0.05) at each TNF-α concentration. (n = 4; *P < 0.05). c Fluorescent micrograph shows fibrin (green) is formed along with platelet aggregates (red) on a fixed endothelium that was pretreated with TNF-α and perfused with recalcified citrated whole blood (left, bar, 200 μm; right, bar, 20 μm)