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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Curr Protoc Hum Genet. 2016 Jul 1;90:21.5.1–21.5.14. doi: 10.1002/cphg.3

Figure 2. Generation of NKX2.1+ neuron progenitors from hES/iPS cells.

Figure 2

(A) Schematic of the differentiation protocol for the generation of hypothalamic progenitors from hES/iPS cells. (B) Live cell imaging on 1, 4, 6, 8, 10, 12 day differentiated cells during the first 12 days of hypothalamic neuron differentiation. Scale bar, 100 μm. (C) Immunocytochemistry analysis of neuron progenitor markers in 12 days differentiated NKX2.1 W/GFP-hES cells. NKX2.1, Nestin, PAX6, FOXG1, SOX1, MASH1 were analyzed. GFP is an endogenous signal indicating the expression of NKX2.1. Scale bar, 200 μm.