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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Curr Protoc Hum Genet. 2016 Jul 1;90:21.5.1–21.5.14. doi: 10.1002/cphg.3

Figure 3. Differentiation of hypothalamic ARC-like neurons from NKX2.1+ progenitors.

Figure 3

(A) Schematic of the differentiation protocol for generating hypothalamic ARC-like neurons from day 12 neuronal progenitors; (B) Live cell imaging during neuron differentiation on days 12, 14, 16, 20 and 24 on Poly-L-Ornithine and laminin coated plate; Scale bar, 100 μm; (C) Immunocytochemistry of 29 days of differentiated neurons. Neurons were stained with αMSH, β-Endorphin, NPY, Somatostatin and AGRP. Scale bar represents, 100 μm.