Figure 5. Synthetic PKA regulation of Ca_V1.2 in HEK293 cells.

(a) Schematic of full-length Ca_V1.2 channel, PKA holoenzyme (R and C) and ICDI_1.4 peptide, along with β_1b tethered CaM and AKAP79. (b) Time course of normalized peak current (top) and CDI (middle), from whole-cell Ca²⁺ currents, evoked every 30 s by depolarizations to 20 mV. Currents were normalized to baseline. CDI was measured as 1−r₁₀₀. All error bars indicate ±s.e.m. (n=6 cells). Corresponding current waveforms are displayed below.