TABLE 2.
Validation criteria for MPS proceduresa
| Validation criteria |
|---|
| Extraction of DNA (or RNA) |
| Quality |
| Purity |
| Enrichment |
| PCR |
| Capture |
| Library preparation |
| Multiplexing |
| Markers |
| Samples |
| Sequencing |
| Data analysis |
| Raw data processing |
| Quality scores |
| Alignment |
| Variant calls |
| Reference materials |
| Test materials |
| Controls |
| Databases |
| Test materials |
| Inferences of results |
| Bioinformatics |
| Software tools |
| Data management |
| Data storage |
| Interpretation |
| Taxonomic assignment |
| Abundance |
| Organism classification |
| Community structure |
| Standard operating protocols |
| Reporting |
a
Based on data from reference 105.