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. 2016 Jul 14;2016:7651024. doi: 10.1155/2016/7651024

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Copyright © 2016 Agnieszka Pozdzik et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Distribution of the B lymphocyte subpopulations after B cells depleting therapy with rituximab as evaluated by fluorescence-activated cell sorter (FACS) analysis of peripheral blood mononuclear cells (PBMC). (a) Dot-plots representing the expression of IgD (FITC) and CD38 (AA750) monitored in our patient with corticosteroid-resistant PLA2R1 related membranous nephropathy during 15 months after first rituximab injection. Graphs representing the kinetics of absolute cells number per mm³ of (b) plasmablasts (CD3⁻CD19⁺CD20⁻IgD⁻CD27^highCD38^high), (c) memory B cells (CD3⁻CD19⁺CD20⁺IgD⁻CD27⁺CD38⁻), and (d) naïve (CD3⁻CD19⁺CD20⁺IgD⁺CD27⁻CD38^low) B cells. (e) IgG4⁺ B cells (absolute cells number per mm³) and anti-PLA2R1 antibody assessed in serum expressed in IU/mL.