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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Surg Res. 2016 Mar 3;204(1):164–175. doi: 10.1016/j.jss.2016.02.030

Figure 1. Effects of enteroid seeding substrate on the production of TEI.

Figure 1

A) Percent positivity of TEI. B) Neomucosal length of TEI. Enteroids were seeded onto PGA scaffolds after 11-14 days in culture using either complete enteroid culture medium or Matrigel. Scaffolds were then implanted into the peritoneal cavity of NOD/SCID mice four weeks after which they were explanted and analyzed using H&E staining. Positivity was defined as the presence of simple columnar epithelium consistent with intestinal mucosa. Neomucosal length was quantified on a representative section using ImageJ software by tracing the basal surface of all simple columnar epithelium. n = 15 for both media and Matrigel groups. *p ≤ 0.05.