Figure 3. Effect of enteroid seeding density on the production of TEI.

A) Percent positivity of TEI. B) Neomucosal length of TEI. Enteroids were seeded to PGA scaffolds after 11-14 days in culture at 10, 20, or 30 enteroids/mm³ of scaffold. The scaffolds were then implanted into the peritoneal cavity of NOD/SCID mice four weeks after which they were explanted and analyzed using H&E staining. Positivity was defined as the presence of simple columnar epithelium consistent with intestinal mucosa. Neomucosal length was quantified on a representative section using ImageJ software by tracing the basal surface of all simple columnar epithelium. n = 15 for 10 and 20 enteroids/mm³; n = 13 for 30 enteroids/mm³. *p ≤ 0.01.