Tobacco smoke induced alterations in the expression of epithelial-mesenchymal transition markers. (A) Tobacco smoke reduced mRNA levels of E-cadherin, ZO-1, and CK5, and elevated mRNA levels of Snial-1, Vimentin, and N-cadherin in the stomach of mice exposed to tobacco smoke for 12 weeks; (B) Tobacco smoke decreased the protein levels of E-cadherin, ZO-1, and CK5, and increased the protein levels of Snial-1, Vimentin, and N-cadherin in the stomachs of mice; (C) Densitometric analyses of Western blotting; (D) Tobacco smoke decreased E-cadherin protein expression and increased Vimentin protein expression shown by immunohistochemical staining. Data are expressed as mean ± SD. ** p < 0.01, compared with control. FA = filtered air; TS = tobacco smoke.