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| time (min) | aqueous (%) | organic (%) | flow rate (mL/min) | gradient curve |
|---|---|---|---|---|
| 0 | 20 | 80 | 0.3 | 1 |
| 30 | 20 | 80 | 0.3 | 6 |
| 32 | 80 | 20 | 0.3 | 6 |
| 40 | 95 | 5 | 0.3 | 6 |
| 42 | 95 | 5 | 0.3 | 6 |
Chromatographic separation of metabolites was carried out using an organic (1% v/v formic acid in acetonitrile)/aqueous (1% v/v aqueous formic acid) mobile phase run over a period of 46 min. Table data show the percentage of each mobile phase at particular time points, flow rate, and gradient curve conditions.
