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. 2016 Jul 27;16:548. doi: 10.1186/s12885-016-2416-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cell proliferation assay. Cells (5 × 10³ cells per well) were seeded on 96-well plates, and cultured for 48 and 72 h. Cell growth was evaluated by MTT assay (OD490; absorbance at 490 nm). The growth rate of HSC2/hOCT3/4-shp53-F + hSK + hUL was significantly higher than that of HSC2 and HSC2/EGFP at 48 and 72 h after seeding. Error bars represent the standard deviation of the mean of three independent experiments