. 2016 Jul 28;12:33. doi: 10.1186/s13223-016-0135-z

Table 1.

Identification of allergens from Lantana camara pollen grains using MALDI TOF/TOF

SN	Identified protein^d	Organism	Accession no.	Identified peptides^b	E value^a	Expt. MW/pI	Theor. MW/pI	Score	UP^b	Seq. cov. (%)
1.	Actin	Glycine max	CAA23728.1	KIWHHTFYNELRV	0.0019	45.6/5.8	41.56/5.23	64	2	3.45
1.	Actin	Maurandya barclaiana	AAL89713.1	KSFELPDGQVITIGNERF	6.2e−05	45.6/5.8	19.996/5.02	78	2	9.94
2.	Actin	Glycine max	CAA23728.1	KIWHHTFYNELRV	0.00017	45.6/5.8	41.56/5.23	69	3	3.45
		Chlorella vulgaris	AAB07499.1	KSYELPDGQVITIGNERF	1.3e−05		23.18/4.89	83		8.78
		Phaseolus vulgaris	AGV54465.1	RTTGIVLDSGNGVSHTVPIYEGYALPHAILRL	0.0037		41.66/5.39	50		8.48
3.	TLP; Ole e 13	Olea Europea	E3SU11.1	RCPDAYSYPQDDPTSLFTCPAGTNYRV	1.3	19.4/4.2	24.7/5.1	31	2	11.94
3.	HP	Erythranthe guttata	EYU38379.1	RCTAPINEQCPNELRA	0.075		24.9/5.3	42	2	6.95
4.	F1ATPase	Pisum sativum	BAA20135.1	KTEHFLPIHRE	0.014	51.8/5.5	60.1/6.6	52	3	1.96
	ATP Synthase subunit beta	Nicotiana plumbaginifolia	CAA26620	RFTQANSEVSALLGRI	0.027		59.8/5.9	48		2.85
	ATP Synthase subunit beta	Tricum aestivum	CAA52636.1	RLVLEVAQHLGENVVRT	0.013		59.24/5.5	50		3.07
5.	ATP Synthase beta subunit	Tricum aestivum	CAA52636.1	RLVLEVAQHLGENVVRT	0.046	51.8/5.6	59.2/5.56	44	2	3.07
5.	ATP Synthase beta subunit	Nicotiana plumbaginifolia	CAA26620.1	RDAEGQDVLLFIDNIFRF	0.0077	51.8/5.6	59.85/5.95	56	2	3.21
6.	ATP Synthase beta subunit	Triticum aestivum	CAA52636.1	RLVLEVAQHLGENVVRT	0.43	51.84/	59.24/5.56	38	2	3.07
6.	ATP Synthase beta subunit	Nicotiana plumbaginifolia	CAA26620.1	RDAEGQDVLLFIDNIFRF	0.29	5.7	59.85/5.95	40	2	3.21
7.	TLP; Ole e 13	Olea Europea	E3SU11.1	RCPDAYSYPQDDPTSLFTCPAGTNYRV	5.1e−13	21.85/6.5	24.72/5.10	150	3	11.94
	Predicted TLP	Solanum Lycopersicum	XP_004252644.1	RLDSGQSWQVNVAARVRAR	NA^c	21.85/6.5	45.53/4.95			4.55
	PRP	Solanum tuberosum	XP_006364119.1	NQQCPNELRN	NA^c		25.00/5.31			4.4
8.	TLP; Ole e 13	(Olea Europea)	E3SU11.1	RCPDAYSYPQDDPT SLFTCPAGTNYRV	5.9e−06	19.28/6.159	24.72/5.10	79	2	11.94
8.	HP	Erythranthe guttata	EYU38385.1	RCTAPINEQCPNELRA	0.018	19.28/6.159	81.11/8.03	48	2	2.2
9.	Predicted GAD 1- like	Glycine max	XP_003544967.1	KVLHELDLLPARV	0.94		52.685/5.831	34	2	2.58
9.	GAD	Nicotiana tabacum)	AAM48129.1	HNEFELSDTLR	NA^c		52.685/5.831		2	2.21

^aProbability score at the <0.05 level

^bUP, unique peptides have been identified by MASCOT search against NCBI database

^cManual de novo sequencing has been performed for the respective peptide sequence

^dIdentified proteins: actin, TLP, thaumatin like protein; HP, hypothetical protein MIMGU_ngvla013131 mg; PRP, pathogenesis related protein, GAD, glutamic acid decarboxylase