Fig. 1.

Flow chart for the experimental design of this study. Three datasets, 180 M, 360 M and 600 M, were used for each of the four assembly strategies described in the text. Subsequently, all fourteen assemblies were assessed by the metrics contig number and length distribution, percent paired-end reads mapped back to the assembly and gene model representation. De novo stands for de novo assembly, Ref-based for reference-based re-assembly, TransPS for transcriptome post-scaffolding, and G. guided for genome-guided assembly