Table 1.
Summary statistics for transcriptome assemblies from multiple strategies
| Assembly Strategy | Dataset used | # of contigs | Median length (bp) | Median %ID vs. dipteran | Median reference coverage vs. dipteran | Median %ID vs. aegypti | Median reference coverage vs. aegypti |
|---|---|---|---|---|---|---|---|
| De novo assembly | 180Ma | 96,980 | 414 | 90.95 % | 98.37 % | 91.32 % | 98.38 % |
| 360 M | 141,390 | 394 | 90.57 % | 98.21 % | 91.02 % | 98.14 % | |
| 600 M | 176,168 | 385 | 90.43 % | 98.37 % | 90.97 % | 98.33 % | |
| Reference-based re-assembly | 180 M | 89,260 | 399 | 91.04 % | 98.46 % | 91.45 % | 98.46 % |
| 360 M | 130,168 | 382 | 90.70 % | 98.22 % | 91.15 % | 98.26 % | |
| 600 M | 162,168 | 373 | 90.57 % | 98.39 % | 91.10 % | 98.45 % | |
| Transcriptome Post-Scaffolding | 180 M | 11,796 | 1,783 | 92.18 % | 99.40 % | 92.57 % | 99.40 % |
| 360 M | 12,027 | 1,854 | 91.97 % | 99.40 % | 92.41 % | 99.39 % | |
| 600 M | 12,117 | 1,888 | 91.90 % | 99.41 % | 92.39 % | 99.41 % | |
| Genome-guided assembly using Ae. albopictus genome | 180 M | 43,537 | 1,091 | 90.95 % | 97.56 % | 91.28 % | 97.78 % |
| 360 M | 57,524 | 1,038 | 90.73 % | 97.57 % | 91.05 % | 97.77 % | |
| 600 M | 68,977 | 1,000 | 90.56 % | 97.76 % | 91.03 % | 97.93 % | |
| Genome-guided assembly using Ae. aegypti genome + RAb | 180 M | 18,999 | 1,426 | 100.00 % | 100.00 % | 100.00 % | 100.00 % |
| Genome-guided assembly using Ae. aegypti genome-RA | 180 M | 13,230 | 348 | 100.00 % | 42.47 % | 100.00 % | 42.89 % |
adataset representing approximate number of millions of read pairs used in the corresponding assembly strategy
bRA refers to reference annotation