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. 2016 Jul 28;7:97. doi: 10.1186/s13287-016-0359-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Quantitative evaluation of osteogenesis. a Quantification of Alizarin Red staining by dissolving the dye and subsequent absorption measurement. b Alkaline phosphatase (ALP) activity in cells cultured in osteogenic medium. ALP activity (mU μmol p-nitrophenol released per min) was normalized for protein. c Calcium concentration determinations were possible only for DPSC samples and AF-MSC samples. The data are expressed as mean ± SD and are representative of two independent experiments, each performed in triplicate (DPSC = 2 donors, ADSC = 2 donors, AF-MSC = 2 donors). *p < 0.05; **p < 0.01; ***p < 0.001. ADSC adipose tissue mesenchymal stromal cell, AF-MSC amniotic fluid mesenchymal stromal cell, DPSC dental pulp mesenchymal stromal cell, MSC mesenchymal stromal cell, sh short hairpin