Table 2.
Morphology changing of A549 cells after incubating with BLM at various concentrations in serum-free medium for 24 h or 48 h. Every cell saple chose six images in same scale. (200×) SPOT software was used to measure about 20 cells per image randomly. And the mean values ± SD are presented. (*P≤0.05), (**P≤0.01).
| Sample | Time | Area (μm2) | Perimeter (μm) | Roundness | |
|---|---|---|---|---|---|
| Control | 24h | 112.56±26.15 | 187.91±32.66 | 0.65±0.12 | |
| 48h | 106.64±24.75 | 182.21±9.68 | 0.64±0.12 | ||
| BLM(μg/mL) | 10 | 24h | 184.71±28.97* | 237.26±27.76* | 0.66±0.11 |
| 48h | 275.85±65.18** | 333.84±59.77** | 0.51±0.13* | ||
| 20 | 24h | 197.37±46.71* | 261.45±36.62* | 0.58±0.09* | |
| 48h | 219.94±58.99** | 338.61±78.58** | 0.41±0.12** | ||
| 50 | 24h | 225.96±72.42* | 280.46±57.39** | 0.58±0.12 | |
| 48h | 502.53±137.14** | 556.53±109.51** | 0.33±0.12** | ||