Table 1.
Antibodies used in western-blot (WB) and immunohistochemical (IHC) analyses
| Primary antibodies | Source/Reference catalog | WB | IHC |
|---|---|---|---|
| rabbit anti-ataxin-7 | Thermo Scientific/(PA1-749) | 1:2000 | 1:5000 |
| rabbit-anti-ubiquitin | Dako/(Z0458) | – | 1:2000 |
| mouse anti-ataxin-7 (1C1) | Provided by Dr Didier Devys | 1:5000 | 1:5000 |
| mouse anti-Calbindin D-28 K | Swant/(300) | – | 1:4000 |
| mouse anti-microtubule-associated Protein 2 (MAP2) | Sigma-Aldrich/(M1406) | – | 1:2000 |
| mouse anti-synaptophysin | Abcam/(ab32594) | – | 1:250 |
| mouse anti-SNAP-25 | Abcam/(ab24737-250) | – | 1:1000 |
| rabbit-anti-PSD-95 | Abcam/(ab18258) | – | 1:1000 |
| rabbit anti-Glial Fibrillary Acidic Protein (GFAP) | Dako/(Z0334) | – | 1:2000 |
| rabbit anti-ionized calcium binding adapter molecule 1 (Iba1) | Wako/(019-19741) | – | 1:3000 |
| rat anti-CD11b/Mac-1 | AbD Serotec/(MCA74G) | – | 1:1000 |
| rabbit anti-GFP | Abcam/(ab6556) | – | 1:5000 |
| rabbit cleaved caspase-3 (Asp175) | Cell Signaling/(9664S) | – | 1:500 |
| mouse anti-neurofilament 70 KDa | Millipore/(MAB1615) | – | 1:1000 |
| rabbit anti-phospho-TDP-43 (pS409/410) | Cosmo Bio/(CAC-TIP-PTD-P01) | 1:1000 | 1:3000 |
| rabbit anti-TARDBP (TAR DNA-binding protein 43) | Proteintech/(10782-2-AP) | – | 1:3000 |
| Mouse anti-FUS/TLS(4H11) (Fused in Sarcoma/Translocated in Sarcoma) | Santa Cruz/(sc-47711) | 1:1000 | 1:500 |
| rabbit-anti-MBNL1 (Muscleblind-like protein 1) | Santa Cruz/(sc-47740) | 1:1000 | 1:500 |
| rabbit-anti-MBNL2 (Muscleblind-like protein 2) | Abcam (ab171551) | 1:2000 | 1:500 |
| rabbit-anti-MBNL2 | Abcam (ab108519) | – | 1:500 |
| rabbit anti-tubulin | Abcam/(ab134185) | 1:5000 | – |
| rabbit anti-iNOS (Nitric oxide synthase) | Cayman Chemicals/(160862) | – | 1:400 |
| rat anti-CD68 | Abd serotec/(MCA1957) | – | 1:500 |
| rabbit anti-TGF-ß (Transforming growth factor beta) | Abcam/(ab66043) | – | 1:1000 |
| rabbit anti-TREM2 (Triggering receptor expressed on myeloid cells 2) | R&D systems/(MAB1729) | – | 1:500 |
| goat anti-vimentin | Santa Cruz/(sc-7557) | – | 1:500 |
| rabbit anti-p62/SQSTM1 (sequestosome 1) | BD Biosciences/(610832) | – | 1:500 |
| mouse anti-actin | Abcam/(ab9484) | 1:4000 | – |
| mouse anti-SMI-25 | Covance/(SMI-25R) | – | 1:2000 |