Fig. 3.
Effect of Kiom-18 extract on the degradation of p27Kip1 expression stimulated by PDGF-BB. Quiescent VSMCs cultured in serum-deprived medium were stimulated with 25 ng/mL PDGF-BB, and the inhibitory effect in the presence of Kiom-18 extract (5–50 μg/ml) on p27Kip1 expression was examined using SDS-PAGE followed by immunoblotting (a), and immunofluorescence (b). Total β-actin and the fluorescence level of PI were used for normalization. The immunoblotting (a) and immunofluorescence (c) were analyzed using densitometry; the values represent the percentage of the stimulated control or the non-stimulated control. The results are an average of three similar, independent experiments and are expressed as the mean ± SEM. Significant differences compared to the control stimulated by PDGF-BB are shown by **p < 0.01