Figure 2. ZEP gene knockout via DNA-free RGEN RNPs delivery.

(a) Schematic diagram of the reversible xanthophyll cycle in green algae. (b) Alignment of wild type (WT) and the mutant line DNA sequences at the ZEP locus. The 20-bp target sequence is underlined and the PAM sequence is shown in red. The column on the right indicates the number of inserted (+). (c) Quantification of pigment content and Chl a to Chl b ratios of wild type (WT) and the ΔZEP mutant lines. Cells were grown TAP media under low light (50 μmol photons m⁻² s⁻¹) conditions. Vio, violaxanthin; An, antheraxanthin; Zea, zeaxanthin. Data are the average and SE from three replicates.