Table 1. Quantification of pigment content and photosynthetic activities of the wild type, ΔZEP, ΔCpFTSY and ΔZEP/ΔCpFTSY mutants.
WT | ΔZEP | ΔCpFTSY | ΔZEP/ΔCpFTSY | |
---|---|---|---|---|
Vio* (fmol per cell) | 0.1043 ± 0.0010 | ND | 0.0551 ± 0.0003 | ND |
An* (fmol per cell) | 0.0085 ± 0.0002 | ND | 0.0033 ± 0.0002 | ND |
Zea* (fmol per cell) | 0.0062 ± 0.0005 | 0.1376 ± 0.0007 | 0.0056 ± 0.0002 | 0.0827 ± 0.0009 |
Total Chl* (fmol per cell) | 1.66 ± 0.01 | 1.41 ± 0.03 | 0.51 ± 0.01 | 0.60 ± 0.01 |
Chl a/b ratio* (fmol:fmol) | 2.24 ± 0.02 | 2.27 ± 0.02 | 6.22 ± 0.15 | 6.14 ± 0.09 |
Quantum yield (relative units) | 100 ± 3.63 | 109.05 ± 14.36 | 117.09 ± 8.10 | 109.85 ± 8.59 |
Pmax (mmol oxygen (mol Chl)−1 s−1) | 48.93 ± 0.76 | 61.4 ± 2.12 | 143.28 ± 3.24 | 94.77 ± 2.21 |
*Pigments were measured under low-light (50 μmol photons m−2 s−1) conditions. Vio, violaxanthin; An, antheraxanthin; Zea, zeaxanthin.
Data are the average and SE from three replicates.