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. 2016 Jun 24;17(7):959. doi: 10.3390/ijms17070959

Table 1.

Primers used in this study. The underline sequence are mutation site.

Primer Name Primer Sequence 5′–3′ Application
Sox5 F TGGCAATGGGATCAGGGAAC Real time PCR (Polymerase Chain Reaction)
Sox5 R ATCATACCCATGAGCTGCCG Real time PCR
Sox5 3′ UTR F CCGCTCGAGGCTGACAAATAGACCTCAGCC Luciferase reporter-wt
Sox5 3′ UTR R ACGCGTCGACTGTTGGCACTGTGTACCTGA Luciferase reporter-wt
Sox5-3′ UTR-mut F GAAGAAGAGATCGAAATTTCGTATGGGTTAAACAGTGCC Luciferase reporter-mut
Sox5-3′ UTR-mut R GGCACTGTTTAACCCATACGAAATTTCGATCTCTTCTTC Luciferase reporter-mut
miR-21a F ACACTCCAGCTGGGTAGCTTATCAGACTGAT Real time PCR
Universal Primer TGGTGTCGTGGAGTCG Real time PCR
U 6 F CTCGCTTCGGCAGCACA Real time PCR
U 6 R AACGCTTCACGAATTTGCGT Real time PCR
miR-21a R CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGTCAACAT Real time PCR
MITF F CAGCAACGAGCTAAGGACC Real time PCR
MITF R GGATGGGATAAGGGAAAGT Real time PCR
18S-F GAAGGGCACCACCAGGAGT Real time PCR
18S-R CAGACAAATCACTCCACCAA Real time PCR
TYR-F ACTTACTCAGCCCAGCATCC Real time PCR
TYR-R AGTGGTCCCTCAGGTGTTCC Real time PCR