Figure 4.

Schematic localization of CAPN10/GPR35 loci. (A) Genomic organization of CAPN10 (left) and GPR35 (right) genes according to RefSeq and Ensembl annotations. The annotation shown in black represents the ab initio transcriptome of HEK293 cells as reconstructed from RNA-Seq data. Boxes indicate the exons. The blue arrow indicates the genomic localization of the polymorphism. A new transcript, automatically annotated in Ensembl database, links the two genes; (B) agarose gel electrophoresis picture in HEK293 cell line treated (or not) with cycloheximide (CHX) at growing concentrations as indicated above the gel image. The newly described transcript is indicated by the black arrow. PPIA gene, shown below, was used as a housekeeping control gene.