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Exogenous HA promotes the expression of transcription factor upstream of ECM protein under hypoxic conditions. Rat articular cartilage was cultured in the presence of 4000 µg/mL HA for 72 h under hypoxia (1% oxygen). SOX9 mRNA expression was analyzed by real-time RT-PCR (A); and SOX9 protein expression was analyzed by Western blotting (B,C). ** p < 0.01.
