Figure 2.

Effects of the PIGA ligands on pregnenolone production. C6 glioma cells (A) and U87MG cells (B) were incubated with different PIGA ligands (40 µM) for 2 h at 37 °C. The pregnenolone amounts were quantified by a competitive enzyme-linked immunosorbent assay. The data are expressed as percentage of pregnenolone production compared to the control, which was set to 100%, and represent the means ± SEM of three different determinations performed in duplicate. The statistical analysis was performed using one-way ANOVA and Bonferroni’s post-test; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control.