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. 2016 Jul 12;17(7):1051. doi: 10.3390/ijms17071051

Figure 4.

Figure 4

The effects of STC-1 on the mRNA levels of ROS-mediated molecules. Among 16 mice, eight mice were used to create RIRI models as a model group and eight mice were used as a sham group. Renal progenitor cells were isolated from the kidney cortex of RIRI model and sham mice after 16-h surgery. For the kidney cells isolated from eight model mice, they were further subdivided into three groups based on different treatment options (model group (MG); STC-1 expression group (MSG), the mice were transfected with STC-1 gene to overexpress STC-1; and STC-1 shRNA group (MSShG), the mice were transfected with STC-1 shRNA to knockdown STC-1). In the same way, the kidney cells from 8 sham mice were subdivided into another three groups based on different treatment options (sham group (SG); STC-1 expression group (SSG), the mice were transfected with STC-1 gene to overexpress STC-1; and STC-1 shRNA group (SSShG), the mice were transfected with STC-1 shRNA to knockdown STC-1). The mRNA levels were measured from the same part of renal tissues obtained after 4-h surgery: (A) the changes of mRNA levels of STC-1 in different groups; (B) the changes of mRNA levels of PKC-α in different groups; (C) the changes of mRNA levels of ERK in different groups; (D) the changes of mRNA levels p53 in different groups; (E) the changes of mRNA levels of IKK in different groups; (F) the changes of mRNA levels of MEKK-1 in different groups; (G) the changes of mRNA levels of JNK in different groups; (H) the changes of mRNA levels of ASK-1 in different groups; (I) the changes of mRNA levels of caspase-3 in different groups; and (J) the changes of mRNA levels of NF-κB in different groups. All data were normalized to actin, and presented as the mean values ± S.D. * p < 0.05 compared to the model group and # p < 0.05 compared to the sham group.