Figure 2.

Conditioned medium (CM) from bone-marrow-derived mesenchymal stem cells (BM-MSC) increases aquaporin 1 (AQP1) levels in SNU-398 and osteosarcoma cells (U2OS) tumor cell lines. (A) Western blot analysis of AQP1 expression in U2OS and SNU-398 after treatment with BM-MSC-CM for 24 h. The equal protein loading was evaluated by using actin. Representative images from three independent studies; (B) AQP1 levels were represented as fold change respect to 10% fetal bovine serum (FBS), which was arbitrarily determined to be 1. Results are expressed as means ± SD.