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. 2016 Jul 12;17(7):1117. doi: 10.3390/ijms17071117

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of lymphocytes reacted with MAb 2D8. A1, B1 and C1 represented lymphocytes in blood, spleen, and pronephros gated (R) on a forward scatter (FSC) versus side scatter (SSC) dot plot, respectively; A2, B2 and C2, rOmpC-injected fish, combined (smoothed) Fluorescein isothiocyanate (FITC) fluorescence histogram of gated lymphocytes (R) showing the percentages of sIg+ lymphocytes (scale of M) in blood, spleen, and pronephros at week 4 after immunization, respectively.