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Serum antibodies against E. tarda in vaccinated fish. Sera were collected from three fish before immunization and at week 1, 2, 3, 4, 5 and 6 after immunization, serum antibodies against E. tarda were determined by ELISA. Results are expressed as means ± SEM (n = 3). Different letters “a” and “b” on the bars represent the statistical significance (p < 0.05) compared to each other at same time point.
