Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 28;6:30622. doi: 10.1038/srep30622

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Co-immunoprecipitation was performed using antibodies against Amot, AmotL1 and AmotL2 in MS-1 cells. AmotL2 was shown to be associated with VE-cadherin whereas AmotL1 could not be immunoprecipitated with VE-cadherin or N-cadherin. (b) Western blot analysis of junctional protein expression in VE-cadherin^+/+, ^+/− and ^−/− endothelial cells (c) Co-immunoprecipitation of N-cadherin with AmotL1 in VE-cadherin^−/− endothelial cells. (d) Immunoprecipitation analysis of endothelial cells siRNA depleted of VE-cadherin (e) Immunofluorescent staining of AmotL1 (in green) and N-cadherin (in red) in VE^+/+ and VE^−/− cells showing co-localization in cellular adhesion junctions in VE-cadherin deficient endothelial cells. Nuclei were visualized by DAPI staining (in blue). Size bar, 10 μm.