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. 2016 Jul 28;6:30542. doi: 10.1038/srep30542

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative midgut sections (Supplementary Fig. 3) were from WCR 3^rd instars treated with H₂O (top panel), gus dsRNA (middle panel) and dvssj1 frag1 (bottom panel) at 50 ng μl⁻¹ for 48-h. All treatments were hybridized with the dvssj1 probe and an RNAscope^® negative control probe (Bacillus subtilis dihydrodipicolinate reductase (dapB) gene). Expression of dvssj1 mRNA is observed in midgut epithelium cells (ep) and oenocyte cells (o) of H₂O and control gus treatment. Knockdown of dvssj1 mRNA in midgut epithelium cells (ep) and oenocyte cells (o) is observed in larvae treated with dvssj1 dsRNA (bottom panel). No clear presence of dvssj1 mRNA in fat body cells (f) and dvssj1 dsRNA in midgut lumen was observed (arrow). Images were captured at 40× magnification with 100 μm scale bars.