Table 1. Diet-based results of WCR dsRNA screening.
dsRNA Name | Length (bp) | Relative to orf | Primary scores | 7d LC50, ng μl−1 | 7d IC50, ng μl−1 | |
---|---|---|---|---|---|---|
Start | End | |||||
dvssj1 FIS | 1156 | −27 | 1131 | 2.8 | n/a | n/a |
dvssj1 frag1 | 210 | −25 | 185 | 2.9 | 0.041 | 0.013 |
dvssj1 frag2 | 145 | −6 | 139 | 3.0 | 0.097 | 0.013 |
dvssj1 frag5 | 502 | −25 | 477 | 2.0 | 0.082 | 0.022 |
dvssj2 FIS | 573 | 2934 | 3506 | 3.0 | 1.699 | 0.272 |
dvssj2 frag1 | 225 | 3301 | 3526 | 2.6 | 0.286 | 0.135 |
dvssj2 frag7 | 162 | 16 | 177 | 2.4 | 0.089 | 0.054 |
Primary scores were the average of eight observations in cDNA-based first-round IVT screening (FIS) or subsequent fragment screening. LC50 and IC50 values in ng μl−1 during a 7-day assay. Target sequences are indicated relative to the first letter of the start codon (ATG) of the open-reading frame (orf).