Table 2. LT50 results of five dsRNA’s in WCR.
| Dose (ng μl−1) | dsRNA | % Mortality (12 days) | LT50 (days) | 95% CI | Grouping* |
|---|---|---|---|---|---|
| 50 | dvssj1 frag1 | 100 | 5.5 | 5.2–5.8 | a |
| dvssj2 frag7 | 100 | 6.3 | 6.0–6.7 | b | |
| dvprotb frag1 | 97.8 | 8.5 | 7.9–9.0 | c | |
| dvpat3 frag13 | 93.5 | 9.0 | 8.4–9.7 | c | |
| dvrps10 frag4 | 97.8 | 9.0 | 8.5–9.7 | c | |
| 5 | dvssj1 frag1 | 100 | 6.6 | 6.2–7.1 | a |
| dvssj2 frag7 | 100 | 7.1 | 6.7–7.6 | a | |
| dvprotb frag1 | 97.9 | 8.4 | 7.8–9.0 | b | |
| dvpat3 frag13 | 91.7 | 9.2 | 8.6–10.0 | bc | |
| dvrps10 frag4 | 83.3 | 10.0 | 9.3–10.7 | c |
LT50 represents time in days that 50 percent of WCR larvae (n=45–48) are killed by dsRNA at concentrations of 5 or 50 ng μl−1. Fragments of five active targets were selected for LT50 determination based on dose response assay results (Table 1 and Supplementary Table 2). LT50 values with different letters are significantly different based on non-overlap of 95% CI (P < 0.05).