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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Placenta. 2016 Jun 11;44:69–79. doi: 10.1016/j.placenta.2016.06.005

Figure 2. The ex vivo expression of TLR2, 4, and 6 was detected in human fetal membranes.

Figure 2

(A) Labeling for TLR 2 (i), 4 (ii), and 6 (iii) was observed along the apical surface of AEC with minor staining of AMC. Arrows point to brown immunolabeling in AMC and AEC. IgG (iv – vi) and no primary antibody controls (vii – ix) are shown for each antibody. AMC = amnion mesenchymal cells, AEC = amnion epithelial cells, CD = choriodecidua. Images were taken at a magnification of 50× and are representative of results seen in n = 4 patients. Western blotting (B) for TLR2, 4, and 6 was performed from amnion layer lysate samples. Each well represents a sample from an individual patient (n = 4) with RBL cells as positive control. (C) Quantitation is shown by relative intensities of expression bands normalized to β-actin.