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. 2016 Jul 28;6:30485. doi: 10.1038/srep30485

Figure 5. Allele-specific genome editing using an allele-specific single-nucleotide insertion in vivo.

Figure 5

(a) DNA sequences targeted by sgRNAs. PAMs are shown in green. The inserted single guanine in the Gx5 allele is shown in red. (b) Evaluation of genome editing. Schemes for genome editing and genotyping PCR are shown in Supplementary Fig. S8. Products of genotyping PCR were cloned, and 13 (sgRNA_Gx4#2) or 14 (sgRNA_Gx5#2) independent clones were subjected to DNA sequencing analysis to identify the targeted alleles. (c) Evaluation of locus binding, as determined by DNA yields of conventional in vivo enChIP. The error bar represents s.e.m. of three enChIP experiments (*t-test P-value < 0.05).