Figure 6. Summary of genome editing and locus binding using sgRNA_Gx4#2 or sgRNA_Gx5#2.

Single-nucleotide skipping [“sgRNA jump (DNA bulge)” for sgRNA_Gx4#2 and “sgRNA bulge” for sgRNA_Gx5#2] can occur between the third and seventh positions 5′ of the PAM for each sgRNA. As a representative, single-nucleotide skipping at the seventh nucleotide 5′ of the PAM is shown for each sgRNA. PAMs are shown in green. The single-guanine insertion in the Gx5 allele is shown in red.