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. 2015 Oct 19;12(3):829–836. doi: 10.1080/21645515.2015.1103405

Figure 2.

Figure 2.

Genotype of HLA-A11/DR1 Tg mice and flow cytometric analysis of transgenic HLA molecules expressed on the surface of mouse immune cells. Genomic DNA purified from HLA-A11/DR1 Tg mice was analyzed by PCR using primers specific for HLA-A11, HLA-DR1, mouse β2m, and H-2 IAβ. (A) Identification of HLA-A11 (upper panel) and HLA-DR1 (lower panel). Tg mice, HLA-A11/DR1 Tg mice; NC, Negative control; PC, Positive control; Blank, dH2O. (B) Identification of mouse β2m(upper panel) and H-2 IAβ (lower panel).Tg mice,HLA-A11/DR1 Tg mice; NC, Negative control; PC, Positive control; F1, Heterozygote control; Blank, dH2O.(C and D) Splenocytes from HLA-A11/DR1 (red histogram), H-2-I/II knockout mice (black histogram), and wild-type C57BL/6 (blue histogram) mice were isolated and stained with a PE-anti-human-HLA-ABC antibody (C) or an FITC-anti-human-HLA-DR antibody (D) to detect HLA-A11 and HLA-DR1 expression, respectively.