Figure 2.

Chemiluminescence counts reflecting reactive oxygen species (ROS) production from PMNs challenged with non-mucoid Pseudomonas aeruginosa clinical strains (516/08, 5762C/07, 39014/06, 40542A/07, and 40542B/07) isolated from 4 non-chronically infected CF patients and a flagella mutant (PAO1172 TTN 183). The chemiluminescence, given in counts per second (CPS), was measured using luminol in a microplate fluorescence reader (Wallac 1420 Victor2, Perkin Elmer) over 60 minutes of phagocytosis. Each panel shows the peak CPS ± SEM of experiments run in duplicate from 3 different donors. *Lowest concentration of S-IgY to give a significant increase in respiratory burst compared to the control (p < 0.05)