Figure 1.

Expression of LdNH36 constructs in P. pastoris X-33 compared with E. coli-expressed wild-type LdNH36, evaluated by Western blot with anti-LdNH36 mouse sera (reduced 4–20% Tris-glycine gel/chemiluminescence detection). Lane 1: 10 µL of wild-type LdNH36 culture supernatant expressed in P. pastoris; Lane 2: 10 µL of LdNH36-dg culture supernatant expressed in P. pastoris; Lane 3: 10 µL of LdNH36-dg2 culture supernatant expressed in P. pastoris; Lane 4: 50 ng (determined by 280 nm absorbance) of purified, His-tagged wild-type LdNH36 expressed in E. coli as control.