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. 2016 Feb 2;12(7):1707–1720. doi: 10.1080/21645515.2016.1139254

Figure 5.

Figure 5.

In-process sample analysis with 4–12% Bis-Tris gels. (A) SDS-PAGE with Coomassie blue staining, (B) Western blot with anti-LdNH36/colorimetric detection), and (C) HCP Western blot with anti-P. pastoris/colorimetric detection. Lanes 1–6 are non-reduced and lanes 7–8 are reduced. Lanes M: molecular weight standard; Lane 1: fermentation supernatant; Lane 2: post-TFF; Lane 3: Capto SP pool; Lane 4: concentrated Capto SP pool; Lane 5 and 8: SEC200 pool low load; Lane 6 and 7: SEC200 pool high load.