Figure 6.

HPLC-SEC and DLS characterization of LdNH36-dg2. (A) HPLC-SEC chromatographs of in-process samples are shown with the molecular weight standard in the top chromatograph and corresponding MW of peaks labeled in kDa.  LdNH36-dg2 is present at retention time of 30 minutes corresponding to a MW of 132 kDa, demonstrating that LdNH36-dg2 is a tetramer in solution. The predicted structure of the molecule as a tetramer is shown in (B) with the mutated glutamines highlighted in red. (C) DLS results of the purified LdNH36-dg2 (SEC200 Pool) are presented as % intensity and demonstrate a similar MW (135 kDa).  The polydispersity is 13.2%, indicating a monodisperse purified LdNH36-dg2.