Figure 4.

The antibody responses elicited by the MV-vectored dengue vaccine. Groups of hCD46 mice (n = 4) were immunized with either 2 × 10⁶ pfu of rMV-EGFP or rMV-TDV by ip injection and boosted 4 weeks later (indicated by arrow). The reciprocal titers of specific IgG to MV (A) and DENV-1 to 4 (B-E) were determined by ELISA. The results are presented as the mean and SD of specific IgG titers. (F) The neutralizing antibody titers against parental MV and the 4 serotypes of DENV were determined by plaque reduction and FRNT, respectively. The reciprocal titer leading to a ≥50% reduction (PRNT₅₀ or FRNT₅₀) is shown. The detection limits for the IgG ELISA or neutralization assay are indicated with a dashed line. Mann-Whitney t-tests were used for statistical analyses, and the significance compared with the rMV-EGFP control is shown.