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. 2015 Oct 30;9(5):376–393. doi: 10.1080/19336896.2015.1105432

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© 2015 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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FIGURE 3. — Immunohistochemical staining of 3 mouse genotypes 24hrs post treatment. Nissl staining of cerebral infarction. Representative sections of wt (A, B, C), Sprn^0/0 (D, E, F), and Sprn^0/0-TgSprn^+/− (G, H, I) mice are shown. Normal tissue is stained deep blue while infarcted tissue with loss of neurons appears clearer (A, D, G). Immunohistochemistry is shown representing the effect of brain ischemia on the expression of protein GFAP (B, E, H) and NeuN (C, F, I). J represents a quantification of the size of the infarct volume in the 3 different genotypes (n = 8, 7, 8, respectively). Values between the 3 genotypes were not significantly different (P = 0.57). The scale bar represents 1mm.