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PrPC interacts with 10 different ligands in cross-mating assay. Bait strain expressing PrPC and the Lac-Z reporter gene were mated with target strains expressing the putative PrPC interactors. X-gal was used to score positive interactions (blue color development). pBait and pTarget were used as a positive control for interaction, since they express known interaction partners. The empty bait vector (pGilda), which expresses only Lex A domain, was used as negative control.
