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Immunoblot analysis of PBC sera. Mitochondrial extract was resolved on SDS-PAGE, transferred to nitrocellulose membrane and probed with AMA positive sera. Note AMA reactivity to PDC-E2, E3BP, BCOADC-E2 and OGDC-E2 (lane A), AMA reactivity to PDC-E2, BCOADC-E2 and OGDC-E2 (lane B), AMA reactivity to PDC-E2 and OGDC-E2 (lane C) and AMA reactivity to OGDC-E2 only (lane D). Note: To minimize background, recombinant proteins are preferred over mitochondrial extracts for AMA detection.
